Class - XII Sample Paper Biotechnology (Theory) 2008

Biotechnology Sample Paper
(Theory)
Class - XII - (2008)

TIME: 3 HOUR
MARK: 70

GENERAL INSTRUCTIONS:

a. This question paper consists of four sections A, B, C and D.    Section A contains 5 questions of 1 mark each. Section B contains 10 questions of 2 marks each. Sections C contain 10 questions of 3 marks each and section D contains 3 questions of 5 marks each.

b. All questions are compulsory.

c. Question numbers 1 to 5 are very short answer type questions, carrying 1 mark each.

d. Question numbers 6 to 15 are short answer type questions, carrying 2 marks each.

e. Question numbers 16 to 25 are also short answer type questions, carrying 3 mark each.

f. Question numbers 26 to 28 are long answer type questions, carrying 5 marks each.

SECTION - A

1.      Write the names of two dyes used in two-dimensional gel electrophoresis (1 )

2.      Why curd is used as pro-biotic? (1 )

3.      Define turbid state (1 )

4.      What is meant by somaclonal variations? (1 )

5.      Name the plant from which agar is isolating (1 )

SECTION - B

6.       How does media helps to regulate pH in animal cell cultures? (2 )

7.       What is the role of curator in Bioinformatics data analysis? (2 )

8.       How is calcium alginate useful in the rapid and mass propagation of elite plant species? (2 )

9.       When irradiated with UV-light, yeast cultures express proteins involved in DNA repair. Indicate Which library you would use to clone the genes for these proteins? (2 )

10.   How the monoclonal antibodies are different from polyclonal antibodies? Write one application of monoclonal antibodies in diagnosis  (2 )

11.   ‘A particular Defence mechanism of bacteria is the basis of recombinant DNA technology’. Explain (2 )

12.   Write four ethical issues of microbial technology  (2 )

13.   Write a short note on cosmids  (2 )

14.    Give at least four steps can be taken to maximize the protein stability during various isolation procedure  (2 )

15.   What are the advantages of using molecular markers over that of morphological markers (2 )

SECTION - C


Diagrammatically represent the steps of Southern hybridization    (3 )

16.   Write notes on 

a) Micro-injection

b) Electroporation

c) Transformation (3 )

17.   Mr. Arvind sequenced the genome of an unknown organism. He wants to know the ancestry of the organism and the homologous sequences. Guide him by giving the procedure of the method  (3 )

18.    Explain three reasons why the use of microbes may be harmful in making product of human conception (3)

19.   Study the following enzyme purification table and answer the question that follow

a)       Which is the yield of active protein from crude extract?

b)       Which step in the purification is least effective. Why?

c)       Which step in the purification is most effective. Why?  (3 )

21. Write a note on edible vaccines 

OR

Diagrammatically differentiate dATP and ddATP (3 )

22. Explain three reasons Why the use of microbes may be harmful in making products of human consumption (3 )

23. How can you obtain virus free potato plant from virus infected plants? Are these plants virus resistant? Why or why not? (3 )

24. Karyotype determination of established cell line is very important. Explain  (3 )

25. Name the enzymes which known as ‘laundry enzymes’. How it is inactivated? How can we increase the stability of the enzymes?  (3 )

SECTION – D

26. What is Proteomics? Explain different types of Proteomics with one examples (5 )

OR

a) Describe the important parts of mass spectrometer with the help of a suitable diagram


b) Explain how proteins are volatilized as well as analysed by mass spectrometer. 

c) Why has this technique become so important for biotechnology    (5 )

27. What is PCR? What is its underlying principle? Explain the steps with the help of suitable diagrams  (5 )

28. What is Proteomics? Explain different types of Proteomics with one example (5 )

--WWW.CBSEPORTAL.COM--