CBSE Class-12 Syllabus 2019-20 (Biotechnology)

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CBSE Class-12 Syllabus 2019-20 (Biotechnology)

An unprecedented growth of human knowledge in the field of Biological Sciences coupled with equally significant developments in the field of technology have brought significant changes into existing social and economic systems. The emerging field of Biotechnology is likely to further enhance the applications of Science and Technology in the service of human welfare. Modern Biotechnology processes encompass a wide range of new products such as antibiotics, vaccines, monoclonal antibodies and many more. Furthermore, developments in recombinant DNA technology have yielded numerous new useful products in the fields of healthcare and agriculture. The present syllabus takes care of all these aspects. Due emphasis has been laid on familiarizing the learners with the fundamental concepts, basic techniques and their applications. It is expected that the knowledge gained through the study of different topics and the skills acquired through the prescribed practical work will make the learners competent to meet the challenges of academic as well as professional courses after studying the subject at senior secondary stage.

Objectives

The broad objectives of teaching Biotechnology at senior secondary level are:
To help the learners know and understand basic facts and concepts of the subject at elementary stage.
To expose the students to different basic processes and basic techniques used in Biotechnology.
To familiarize the learners to understand the relationship of the subject to health, nutrition,
environment, agriculture and industry, etc.
To develop conceptual competence in the learners so as to cope up with professional courses in future career.
To acquaint students with different applications of Biotechnology in everyday life.
To develop an interest in students to study biotechnology as a discipline.

CLASS XII (2019-20)

(THEORY)

COURSE STRUCTURE

One Paper

Max. Marks 70+30
Time: 3 hrs.

Units		No. of Periods	Marks
Unit V	Protein and Gene Manipulation	100	40
Unit VI	Cell Culture and Genetic Manipulation	80	30
	Practicals	60	30
	Total	240	100

Unit-V Protein and Gene Manipulation : 40 Marks 100 Periods
Chapter-1: Recombinant DNA Technology

Introduction, Tool of rDNA technology, Making rDNA, Introduction of recombinant DNA into host cells, Identification of Recombinants, Polymerase Chain Reaction (PCR), Hybridization Techniques, DNA Library, DNA Sequencing, Site-directed Mutagenesis

Chapter-2: Protein Structure and Engineering
Introduction to the world of proteins, 3-D shape of proteins, Structure-Function Relationship in Proteins, Purification of Proteins, Characterization of Proteins, Protein Based Products, Designing Proteins (Protein Engineering)

Chapter-3: Genomics, Proteomics and Bioinformatics
Introduction, Genome, Sequencing Projects, Gene prediction and counting, Genome Similarity, SNPs and Comparative Genomics, Functional Genomics, Proteomics, History of Bioinformatics, Sequences and nomenclature, Information Sources, Analysis using Bioinformatics tools

Unit-VI Cell Culture and Genetic Manipulation 30 Marks 80 Periods
Chapter-1: Microbial Cell Culture and its Applications

Introduction, Microbial Nutrition and Culture Techniques, Measurement and Kinetics of Microbial Growth, Scale-up of Microbial Process, Isolation of Microbial Products, Strain Isolation and
Improvement, Applications of Microbial Culture Technology, Biosafety Issues in Microbial Technology

Chapter -2: Plant Cell Culture and Applications
Introduction, Cell and Tissue Culture Techniques, Applications of Cell and Tissue Culture, Gene Transfer Methods in Plants, Transgenic Plants with Beneficial Traits, Biosafety of Transgenic Plants

Chapter-3: Animal Cell Culture and Applications