CBSE Class-12 Compartment Exam 2020 : Question Paper-Bio Technology

Q1. Why is agar added to the culture medium used for culturing microbes ?

Q2. Pectinase enzyme is needed to isolate single cells from a plant tissue. Why ?  

OR 

 For genetic transformation vector, which two DNA sequences are cloned in the T-DNA region of Ti-plasmid in place of unwanted DNA sequences ? 

Q3. Give an example of a disease caused by the absence of the enzyme adenosine deaminase. 

OR

 What is the cause of thalassemia ? 

4. In recombinant DNA technology, what is an ‘insert’ ? 5. Which database retrieval tool carries information on taxonomic classification of an organism ?

OR 

Name a single gene mutation in humans which follows Mendelian inheritance, having chromosome 7 as its genomic location. 
Q6. Frozen animal cell culture should be thawed in a water-bath maintained at 37C, with moderate shaking. Why ?      
Q7. What are the two limitations of the germplasm conservation through conventional methods ?    
Q8. Polyethylene glycol is a  

(A) Fusogenic chemical  

(B) Callus stimulant 

(C) Electrofusion stimulant 

(D) Differentiation stimulant 

Q9. Which one of the following restriction enzymes produces blunt ends in a DNA fragment ? 

(A) Alu I  

(B) Eco RI   

(C) Bam HI    

(D) Hind III     

Q10. The study of full complement of proteins expressed by a genome is called  

(A) Proteome 

(B) Proteomics 

(C) Genomics

(D) Protein analysis 

Q11. Which of the following is a function of cryoprotective agents ?

(A) Long-term preservation of cultures  

(B) Prevention of cell damage due to ice crystal formation

(C) Prevent formation of inclusion bodies 

(D) To trap the liquid nitrogen 

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Courtesy: CBSE