Class XII
Biotechnology (045)
Time allowed: 3 Hours
Max. Marks: 70

General Instructions:
(i) Question paper contains four sections-A, B, C and D.
(ii) All questions are compulsory
(iii) There is internal choice in all sections. You have to attempt only one of the choices in such questions.
(iv) Question numbers 1 to 6 are very short answer questions, carrying 1 mark each.
(v) Question numbers 7 to 14 are short answer questions, carrying 2 marks each.
(vi) Question numbers 15 to 25 are also short answer questions, carrying 3 marks each.
(vi) Question numbers 26 to 28 are long answer questions, carrying 5 marks each.
(vii) Use of calculators is not permitted. However, you may use log tables, if necessary.

SECTION-A

1. 4 copies of ds DNA are subjected to polymerase chain reaction. How many copies would be obtained after 20 cycles?

                                                OR

1.How can we use LEU 2 gene as a selectable marker?

2. Specify the role of alkaline phosphatase in cloning.

3. Specific activity increases during subsequent steps of a protein purification scheme. How can you relate it with the purity of protein?

4. Animal cells in a culture medium were placed in a regular incubator used for growing bacterial cells. Do you expect the animal cells to grow or not?

5. What is Gene Knock out?

6. What will be the consequence if a protein is having an altered structure?

                                    OR

6.Expand and define PER?

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